Restoring the Oxidase-Like Activity of His@AuNCs for the Determination of Alkaline Phosphatase

doi:10.3390/bios11060174

	Restoring the Oxidase-Like Activity of His@AuNCs for the Determination of Alkaline Phosphatase
	Xiao, Fanfan 1; Yu, Yuting 1; Wu, Yang 1; Tian, Lili 1; Zhao, Guoyan 1; Pang, Hailong1 ; Du, Jie1,2
	2021-06
发表期刊	Biosensors-Basel
ISSN	2079-6374
卷号	11 期号:6
摘要	In this paper, we propose a simple colorimetric method for the sensitive and selective detection of alkaline phosphatase (ALP) activity based on the turn off/turn on oxidase mimic activity of His@AuNCs. His@AuNCs/graphene oxide hybrids (His@AuNCs/GO) were easily obtained using the self-assembly method with poly (diallyldimethylammonium chloride) (PDDA)-coated GO and showed high oxidase-like activity compared with His@AuNCs. We found that the pyrophosphate ion (P2O74-, PPi) could effectively inhibit the oxidase mimic activity of His@AuNCs/GO, and the hydrolysis of PPi by ALP restored the inhibited activity of His@AuNCs/GO, enabling them to efficiently catalyze the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to generate the blue oxidized product oxTMB. The intensity of the color showed a linear dependency with the ALP activity. ALP was detected in the linear range of 0-40 mU/mL with a low detection limit (LOD) of 0.26 mU/mL (S/N = 3). The proposed method is fast, easy, and can be applied to monitor the ALP activity in serum samples accurately and effectively, which suggests its practicability and reliability in the detection of ALP activity in clinical practice.
关键词	His@AuNCs/GO oxidase-like activity PPi ALP colorimetric detection
DOI	10.3390/bios11060174
收录类别	SCIE ; EI
语种	英语
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation
WOS记录号	WOS:000666645000001
出版者	MDPI
EI入藏号	20231213757810
EI主题词	Chlorine compounds
EI分类号	741.1 Light/Optics ; 804 Chemical Products Generally ; 941.4 Optical Variables Measurements
原始文献类型	Article
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.nwnu.edu.cn/handle/39RV6HYL/73548
专题	实体学院_生命科学学院科研机构_甘肃省生物电化学与环境分析重点实验室
通讯作者	Du, Jie
作者单位	1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China; 2.Northwest Normal Univ, Key Lab Bioelectrochem & Environm Anal Gansu Prov, Lanzhou 730070, Peoples R China
第一作者单位	生命科学学院
通讯作者单位	生命科学学院; 甘肃省生物电化学与环境分析重点实验室
第一作者的第一单位	生命科学学院
推荐引用方式 GB/T 7714	Xiao, Fanfan,Yu, Yuting,Wu, Yang,et al. Restoring the Oxidase-Like Activity of His@AuNCs for the Determination of Alkaline Phosphatase[J]. Biosensors-Basel,2021,11(6).
APA	Xiao, Fanfan.,Yu, Yuting.,Wu, Yang.,Tian, Lili.,Zhao, Guoyan.,...&Du, Jie.(2021).Restoring the Oxidase-Like Activity of His@AuNCs for the Determination of Alkaline Phosphatase.Biosensors-Basel,11(6).
MLA	Xiao, Fanfan,et al."Restoring the Oxidase-Like Activity of His@AuNCs for the Determination of Alkaline Phosphatase".Biosensors-Basel 11.6(2021).