DNA binding and in vitro antineoplastic activity of neotype water-soluble Cu()-complexes based on fluorinated benzoylhydrazone porphyrin ligands

doi:10.1016/j.dyepig.2018.12.056

	DNA binding and in vitro antineoplastic activity of neotype water-soluble Cu()-complexes based on fluorinated benzoylhydrazone porphyrin ligands
	Li, Zhenzhen; Zhang, Qian; Liu, Hua; Wang, Guangying; Liu, Jiacheng
	2019-04-01
发表期刊	DYES AND PIGMENTS
ISSN	0143-7208
卷号	163 页码:647-655
摘要	Three water-soluble Cu()-based acylhydrazone porphyrin derivatives (named as CuP1, CuP2 and CuP3) were prepared and isolated successfully. Diversiform physicochemical approaches demonstrated that all of the complexes possess excellent binding affinity with ct-DNA, especially CuP3 exhibited strongest binding affinity than its analogues. Moreover, the antiproliferative properties of all compounds in vitro in human cancer cell line (A549 and H-1975 lung cancer cells, HepG2 human hepatocarcinoma, T47D breast cancer cells) and noncancerous cell line (Hs 578Bst normal breast cells) have also been evaluated by MTT assay. Besides, an extracted method was carried out to determine the cellular uptake ability of three complexes to the tested cancer cell lines. The cytotoxicity and cellular uptake tests revealed that CuP3 exhibited the best antiproliferative activity as well as cellular absorption capacity towards H-1975 cell line. In order to better understand the effects of CuP3 on H-1975 cells, the fluorescence microscopy measurements were employed, the cell membrane damage, nuclear condensation, and apoptotic bodies (brighter fluorescence) were markedly observed after incubated with CuP3 for 48 h, suggesting that CuP3 can induce cell apoptosis on H-1975 cells significantly. In addition, the flow cytometric analysis results illustrated that the complex CuP3 mainly caused an accumulation of cells in the G2 phase of the cell cycle. © 2018
关键词	Binding energy Cell culture Cell death Complexation Copper compounds Cytotoxicity Diseases DNA Fluorescence imaging Fluorescence microscopy Porphyrins Anti-proliferative activities Antineoplastic activity Antiproliferative properties Cell cycle Cell membrane damages Cu complexes DNA binding Flow-cytometric analysis
DOI	10.1016/j.dyepig.2018.12.056
收录类别	EI ; SCIE
语种	英语
WOS研究方向	Chemistry ; Engineering ; Materials Science
WOS类目	Chemistry, Applied ; Engineering, Chemical ; Materials Science, Textiles
WOS记录号	WOS:000457666300079
出版者	Elsevier Ltd
EI入藏号	20185306322109
EI主题词	Phosphorus compounds
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 801.4 Physical Chemistry ; 802.2 Chemical Reactions ; 804.1 Organic Compounds ; 931.4 Quantum Theory ; Quantum Mechanics
原始文献类型	Journal article (JA)
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.nwnu.edu.cn/handle/39RV6HYL/32658
专题	科研机构_甘肃省生物电化学与环境分析重点实验室科研机构_生态环境相关高分子材料教育部重点实验室实体学院_化学化工学院机关部门（群众团体）_国有资产管理处（政府采购办公室、国有资产管理委员会办公室）
通讯作者	Liu, Jiacheng
作者单位	Northwest Normal Univ, Key Lab Bioelectrochem & Environm Anal Gansu Prov, Key Lab Polymer Mat Gansu Prov,Coll Chem & Chem E, Key Lab Ecoenvironm Related Polymer Mat,Minist Ed, Lanzhou 730070, Gansu, Peoples R China
第一作者单位	生态环境相关高分子材料教育部重点实验室
通讯作者单位	生态环境相关高分子材料教育部重点实验室
第一作者的第一单位	生态环境相关高分子材料教育部重点实验室
推荐引用方式 GB/T 7714	Li, Zhenzhen,Zhang, Qian,Liu, Hua,et al. DNA binding and in vitro antineoplastic activity of neotype water-soluble Cu()-complexes based on fluorinated benzoylhydrazone porphyrin ligands[J]. DYES AND PIGMENTS,2019,163:647-655.
APA	Li, Zhenzhen,Zhang, Qian,Liu, Hua,Wang, Guangying,&Liu, Jiacheng.(2019).DNA binding and in vitro antineoplastic activity of neotype water-soluble Cu()-complexes based on fluorinated benzoylhydrazone porphyrin ligands.DYES AND PIGMENTS,163,647-655.
MLA	Li, Zhenzhen,et al."DNA binding and in vitro antineoplastic activity of neotype water-soluble Cu()-complexes based on fluorinated benzoylhydrazone porphyrin ligands".DYES AND PIGMENTS 163(2019):647-655.