Endophytic Stenotrophomonas geniculata KJ-6 via producing antifungal volatile organic compounds effectively control Lanzhou lily postharvest diseases

doi:10.1016/j.fbio.2024.103743

	Endophytic Stenotrophomonas geniculata KJ-6 via producing antifungal volatile organic compounds effectively control Lanzhou lily postharvest diseases
	Ling, Lijun1,2,3 ; Wang, Yuanyuan1 ; Li, Jialin 4; Cheng, Wenting 1; Yue, Rui 1; Feng, Lijun 1; Li, Yao1 ; Zhou, Yongpeng 1; Lu, Lu1
	2024-04
发表期刊	FOOD BIOSCIENCE
ISSN	2212-4292
EISSN	2212-4306
卷号	58
摘要	The endophytic bacterial Stenotrophomonas geniculata KJ-6, was isolated from Arisaematis rhizoma. This strain has the ability to control three postharvest pathogenic fungi of Lanzhou lily (Trichoderma lixii F-2, Talaromyces tumuli F-3, and Fusarium annulatum F-6) by releasing volatile organic compounds (VOCs). The experiment utilized the double-layer sealed bottom plate method. The results demonstrate that KJ-6 effectively inhibits the mycelial growth of F-2, F-3, and F-6, with inhibition rates of 81.59%, 79.87%, and 84.7%, respectively. Moreover, scanning electron microscopy (SEM) revealed abnormal mycelium morphology in the test pathogenic fungi treated with KJ-6 VOCs, characterized by multiple wrinkles, cracks, and distortions. Using five common phytopathogenic fungi as controls, our results demonstrated that the VOCs from KJ-6 exhibited broad-spectrum antifungal effects. Analysis using headspace gas chromatography-ion mobility spectrometry identified three components of the VOCs produced by KJ-6, including 1-propanethiol, isoamyl acetate, and 2-methyl-1-butanol. Antifungal tests revealed that 1-propanethiol plays a crucial role in the inhibitory activity of VOCs. It exhibited inhibitory rates of 64.17%, 72.73%, and 26.71% on the mycelium growth of F-2, F-3, and F-6, respectively. In vivo experiments further confirmed that VOCs can significantly reduce the disease index and decay rate of Lanzhou lily bulbs during storage. Moreover, KJ-6 treatment led to a significant reduction in the degree of browning of lily bulbs. Additionally, it enhanced the activity of defense-related enzymes, improved disease resistance, and extended the shelf life of lily bulbs. As a result, this study offers a theoretical foundation for the biological control of postharvest diseases in Lanzhou lily.
关键词	Stenotrophomonas geniculata KJ-6 Volatile organic compounds (VOCs) Lanzhou lily disease Biological control
DOI	10.1016/j.fbio.2024.103743
收录类别	SCIE
语种	英语
资助项目	Science and Technology Pro- gram of Gansu Province [22CX8GA136]; Key Research and Develop- ment Project of Gansu Province [22YF7NA120]
WOS研究方向	Food Science & Technology
WOS类目	Food Science & Technology
WOS记录号	WOS:001193906500001
出版者	ELSEVIER
原始文献类型	Article
文献类型	期刊论文
条目标识符	https://ir.nwnu.edu.cn/handle/39RV6HYL/95142
专题	实体学院_生命科学学院实体学院_外国语学院附属单位_附属小学机关部门（群众团体）_人力资源交流中心
通讯作者	Ling, Lijun
作者单位	1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China; 2.Northwest Normal Univ, Bioact Prod Engn Res Ctr Gansu Distinct Plants, Lanzhou 730070, Peoples R China; 3.Northwest Normal Univ, 967 Anning East Rd, Lanzhou, Peoples R China; 4.Gansu Vocat Coll Agr, Lanzhou, Peoples R China
第一作者单位	生命科学学院; 西北师范大学
通讯作者单位	生命科学学院; 西北师范大学
第一作者的第一单位	生命科学学院
推荐引用方式 GB/T 7714	Ling, Lijun,Wang, Yuanyuan,Li, Jialin,et al. Endophytic Stenotrophomonas geniculata KJ-6 via producing antifungal volatile organic compounds effectively control Lanzhou lily postharvest diseases[J]. FOOD BIOSCIENCE,2024,58.
APA	Ling, Lijun.,Wang, Yuanyuan.,Li, Jialin.,Cheng, Wenting.,Yue, Rui.,...&Lu, Lu.(2024).Endophytic Stenotrophomonas geniculata KJ-6 via producing antifungal volatile organic compounds effectively control Lanzhou lily postharvest diseases.FOOD BIOSCIENCE,58.
MLA	Ling, Lijun,et al."Endophytic Stenotrophomonas geniculata KJ-6 via producing antifungal volatile organic compounds effectively control Lanzhou lily postharvest diseases".FOOD BIOSCIENCE 58(2024).