Integrated lipidomic and transcriptomic analysis reveals diacylglycerol accumulation in olive of Longnan (China)

doi:10.7717/peerj.15724

	Integrated lipidomic and transcriptomic analysis reveals diacylglycerol accumulation in olive of Longnan (China)
	Hu, Wei1 ; Ma, Junyi1 ; Zhang, Hongjie1 ; Miu, Xin 1; Miao, Xin1 ; Deng, Yu 2
	2023-08-11
发表期刊	PeerJ
ISSN	2167-8359
卷号	11
摘要	Background: Olive (Olea europaea L.) oil accumulate more diacylglycerols (DAG) than mostly vegetable oils. Unsaturated fatty acids-enriched DAG consumption enhanced wellness in subjects. However, the mechanism of DAG accumulation is not yet fully understood.Methods: In this study, gene network of DAG accumulation and fatty acid composition in the two olive mesocarps (Chenggu 32 (CG) and Koroneiki (QJ)) were investigated by integrating lipidome and transcriptome techniques.Results: A total of 1,408 lipid molecules were identified by lipidomic analysis in olive mesocarp, of which DAG (DAG36:3, DAG36:4 and DAG36:5) showed higher content, and triacylglycerols (TAG54:3, TAG54:4) exhibited opposite trend in CG. Specifically, DAG was rich in polyunsaturated fatty acids (especially C18:2) at the sn2 position, which was inconsistent with TAG at the same positions (Primarily C18:1). Transcriptomic analysis revealed that phospholipase C (NPC, EC 3.1.4.3) were up-regulated relative to QJ, whereas diacylglycerol kinase (ATP) (DGK, EC 2.7.1.107), diacylglycerol acyltransferase (DGAT, EC 2.3.1.20), and phospholipid: diacylglycerol acyltransferase (PDAT, EC 2.3.1.158) were down-regulated.Conclusion: We speculated that the non-acyl coenzyme A pathway played a significant role in DAG biosynthesis. Additionally, fatty acyl-ACP thioesterase B (FATB, EC 3.1.2.14), stearoyl [acyl-carrier-protein] 9-desaturase (SAD, EC 1.14.19.2) and omega-6 fatty acid desaturase (FAD2, EC 1.14.19.6) were highly expressed in CG and may be involved in regulating fatty acid composition. Meanwhile, phospholipase A1 (LCAT, EC 3.1.1.32) involved in the acyl editing reaction facilitated PUFA linkage at the sn-2 position of DAG. Our findings provide novel insights to increase the DAG content, improve the fatty acid composition of olive oil, and identify candidate genes for the production of DAG-rich oils.
关键词	Diacylglycerols accumulation Gene expression Lipidomics Transcriptomics Virgin olive oil
DOI	10.7717/peerj.15724
收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001050765500002
出版者	PEERJ INC
原始文献类型	Article
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.nwnu.edu.cn/handle/39RV6HYL/93007
专题	机关部门（群众团体）_招生就业处（考试中心）实体学院_生命科学学院实体学院_马克思主义学院附属单位_附属小学机关部门（群众团体）_就业与校友事务处
通讯作者	Ma, Junyi
作者单位	1.Northwest Normal Univ, Coll Life Sci, Lanzhou, Gansu, Peoples R China; 2.Longnan Acad Econ Forestry, Inst Olive, Wudu, Gansu, Peoples R China
第一作者单位	生命科学学院
通讯作者单位	生命科学学院
第一作者的第一单位	生命科学学院
推荐引用方式 GB/T 7714	Hu, Wei,Ma, Junyi,Zhang, Hongjie,et al. Integrated lipidomic and transcriptomic analysis reveals diacylglycerol accumulation in olive of Longnan (China)[J]. PeerJ,2023,11.
APA	Hu, Wei,Ma, Junyi,Zhang, Hongjie,Miu, Xin,Miao, Xin,&Deng, Yu.(2023).Integrated lipidomic and transcriptomic analysis reveals diacylglycerol accumulation in olive of Longnan (China).PeerJ,11.
MLA	Hu, Wei,et al."Integrated lipidomic and transcriptomic analysis reveals diacylglycerol accumulation in olive of Longnan (China)".PeerJ 11(2023).