The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116

doi:10.1007/s00203-022-03216-x

	The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116
	Ling, Lijun1,2,3 ; Cheng, Wenting 1,2; Jiang, Kunling 1,2; Jiao, Zhenglong 1,2; Luo, Hong 1,2; Yang, Caiyun 1,2; Pang, Mingmei 1,2; Lu, Lu1,2
	2022-10
发表期刊	ARCHIVES OF MICROBIOLOGY
ISSN	0302-8933
卷号	204 期号:10
摘要	The growth of Phytophthora capsica, Rhizoctonia solani, Fusarium graminearum, Fusarium oxysporum and Botrytis cinerea were all inhibited by the fermentation supernatant of Bacillus licheniformis TG116, a biocontrol strain isolated from Typhonium giganteum Engl. previously with broad-spectrum resistance to plant pathogens. The fermentation supernatant of the TG116 has a great stability on temperature and UV, and shows the biological activity of protease and cellulase. The antifungal protease produced by B. licheniformis TG116 was purified to homogeneity by ammonium sulfate precipitation, DEAE Sepharose Fast Flow column chromatography and Sephadex G-50 column chromatography. The inhibition of protease by the three surfactants increased with increasing concentration inhibition. Among these surfactants, EDTA showed the strongest inhibition, with only 25% protein activity at a concentration of 1.1 mmol center dot L-1. Gene amplification verified the presence of a gene fragment of serine protease in the strain TG116. The antimicrobial substance isolated from the fermentation broth of TG116 is a serine protease component.
关键词	Bacillus licheniformis Antifungal protein Chromatography isolation Serine protease Fermentation supernatant
DOI	10.1007/s00203-022-03216-x
收录类别	SCIE
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000849650500001
出版者	SPRINGER
原始文献类型	Article
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://ir.nwnu.edu.cn/handle/39RV6HYL/80320
专题	人力资源交流中心实体学院_生命科学学院机关部门（群众团体）_人力资源交流中心
通讯作者	Ling, Lijun
作者单位	1.Northwest Normal Univ, Coll Life Sci, 967 Anning East Rd, Lanzhou 730070, Peoples R China; 2.Northwest Normal Univ, Bioact Prod Engn Res Ctr Gansu Distinct Plants, Lanzhou 730070, Peoples R China; 3.Northwest Normal Univ, New Rural Dev Res Inst, Lanzhou 730070, Peoples R China
第一作者单位	生命科学学院; 西北师范大学
通讯作者单位	生命科学学院; 西北师范大学
第一作者的第一单位	生命科学学院
推荐引用方式 GB/T 7714	Ling, Lijun,Cheng, Wenting,Jiang, Kunling,et al. The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116[J]. ARCHIVES OF MICROBIOLOGY,2022,204(10).
APA	Ling, Lijun.,Cheng, Wenting.,Jiang, Kunling.,Jiao, Zhenglong.,Luo, Hong.,...&Lu, Lu.(2022).The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116.ARCHIVES OF MICROBIOLOGY,204(10).
MLA	Ling, Lijun,et al."The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116".ARCHIVES OF MICROBIOLOGY 204.10(2022).